The Effect of Rice Bran on Triglyceride Levels and Histopatologic Aorta in Rat (Rattus norvegicus) of High Cholesterol Dietary Model

Abstract

Cholesterol is a steroid compound found in animals and humans. Total cholesterol normal levels in rat is 10-54 mg/dL and triglyceride 26-145 mg/dL. Rice bran has a crude fiber content and antioxidants that can be used to resolve these conditions. This study aims to determine the effect of rice bran as therapy in the rats (Rattus norvegicus) high-cholesterol dietary model towards triglyceride levels and histopathologic of the aorta. In this research, rats (Rattus norvegicus) were divided into 5 groups with 4 repetitions. Group A as a negative control, group B was rat which fed with high cholesterol, group C was high-cholesterol diet model of rat with the 16% rice bran therapy/rat/day, group D was high-cholesterol diet model of rat with the 38% rice bran therapy/rat/day, and group E was high cholesterol diet model of rat with the 57% rice bran therapy/rat/day. Rice bran therapy performed for 21 days. Triglyceride levels were calculated by the GPO-PAP method and aortic histopathologic features were observed with HE staining (Hematoxylin-Eosin). The effect of rice bran on blood triglyceride levels was analyzed by one way ANOVA (Analysis of Variance) with α=5% and aortic histopathologic analysis was qualitatively descriptive. The results showed that rice bran therapy could significantly reduce triglyceride levels (p < 0.01) with a dose of 57%/rat/day to decrease triglyceride levels. Rice bran therapy also repaired aortic tissue histopathology of rat with high cholesterol diet model.