The Rice Bran as Therapy Agent to Decrease the SGOT/SGPT activities and Improve the Histopathology of Liver in White Rat (Rattus norvegicus) Induced by High Cholesterol Diet

Abstract

Hypercholesterolemia is a metabolic disorder on increase of total cholesterol levels in blood exceeding normal values. The high cholesterol levels in the blood will be balance by converting to bile acids. The synthesis of bile acids will produce the excessive free radicals leading to oxidative stress. The oxidative stress cause lipid peroxidation of hepatic cell membranes that will increase SGOT and SGPT activities in the blood and changes in the histology of the liver. The content of crude fiber and antioxidants in rice bran are able to overcome the hypercholesterolemia. This study aimed to determine the effect of rice bran as a therapy in white rats (Rattusnorvegicus) high-cholesterol diet model on SGOT and SGPT activities and histopathologic images of liver. Rats were divided into 5 groups, namely: negative control group, positive control group, rice bran therapy group with dose 16%/rat/day, 38%/rat/day and 57%/rat/day. Rice bran therapy performed for 21 days. SGOT and SGPT activities were determined by spectrophotometry and hepatic histopathologic images were observed with HE (Hematoxylin-Eosin) staining. The levels of SGOT and SGPT were analyzed by one-wayANOVA with α = 5% and histopathology of liver was analyzed descriptively. The results showed that rice bran with dose of 57%/rat/day significantly (p <0.05) can decrease SGOT and SGPT activities. Histopathologic observations showed that antioxidants in rice bran could reduce fatty acids on hepatic histopathology. The dosage of 57%/rat/day showed to decrease SGOT, SGPT activities and can reduce fatty acids on hepatic. It can be concluded that the administration of rice bran can reduce the activities of SGOT, SGPT and improve the histopathology of liver rat induced by high cholesterol diet.