Ethyl Acetate and Butanol Fraction Effect of Citrus japonica Thunb. On Triglyceride Levels In Serum White Male Rats (Rattus norvegicus)

Abstract

Hypertriglyceridemia is condition when there is an increase of triglyceride levels in blood. Hypertriglyceridemia can be increased blood pressure and heart problems. One of the plants can be used as a medicine is kasturi citrus peel (Citrus japonica Thunb.). This study aimed to determine the effect of giving butano fraction and ethyl acetate fraction of kasturi citrus peel (Citrus japonica Thunb.) to triglyceride levels on male white rats (Rattus norvegicus). The parameters are triglyceride levels in serum of male white rat induced with high-fat feed containing quail egg yolk (10 ml/kgWB). This research used 25 male white rats that divided into 5 groups with 5 rats per group. Normal groups are not given any treatment, only standard feed. The negative group was induced by high-fat feed and suspension Na CMC, the positive group was given high-fat feed and atorvastatin suspension 0,9 mg/kgWB and treatment group was given high-fat feed induction and suspension of ethyl acetate and butanol fraction of kasturi citrus peel with doses 250 mg/kgBW, as much 1% of body weight wich given orally. The triglyceride levels are calculating by using colorimetri enzymatic method with a photometer Mindray® (BA-88A) at wavelength 510 nm. The data percentage change in triglyceride levels on day 29 to day 15 obtained were analyzed with one-way Analysis of Variance (ANOVA) and continued with the Post Hoc Tukey test for each fraction. The results showed that giving butanol fraction and ethyl acetate fraction of kasturi citrus peel (Citrus japonica Thunb.) at doses of 250 mg/kg body weight had an effect on the decrease in triglyceride levels in serum of white male rat indicated by a significant difference (p<0.05) with negative control and showed a decrease in triglyceride levels activity that was not significant difference (p>0.05) with atorvastatin and there is no significant difference between anti hypertriglyceride activity of butanol fraction and ethyl acetate fraction (p>0.05)