The Effect of Pluchea indica (L.) Less. Tea on Adipogenesis in 3T3-L1 Adipocytes and Lipase Activity.

Kittipot Sirichaiwetchakoon,Gordon Matthew Lowe,Griangsak Eumkeb,Kanjana Thumanu

doi:10.1155/2018/4108787

Abstract

Obesity and hyperlipidemia are a major problem in the world. Pluchea indica (L.) Less. tea (PIT) is a beverage that has various indications. This study focused on the effect of the PIT on inhibiting adipogenesis of 3T3-L1 cells and pancreatic lipase enzyme activity. The viability of 3T3-L1 cells was not significantly decreased after exposure to 200 to 1000 μg mL−1 PIT compared to controls (p > 0.05). The PIT at 750 to 1000 μg mL−1 exhibited a significantly reduced lipid accumulation compared to the control (p < 0.05). The inhibitory effects of the PIT at 250 to 1000 μg mL−1 on lipase activity were significantly increased compared to control (p < 0.05). The FTIR results showed that the integrated areas of lipids, proteins, nucleic acids, glycogen, and carbohydrates of the PIT-treated 3T3-L1 adipocytes were significantly lower than the untreated 3T3-L1 adipocytes (p < 0.05). These findings may indicate that the PIT is not only capable of inhibiting lipids and carbohydrate accumulation in adipocytes but also has a potential to inhibit pancreatic lipase activity. So, the PIT may be further developed to the novel lipid-lowering herbal supplement for the management of overweight or obesity.

Highlights

The global prevalence of obesity is increasing worldwide rapidly [1]
During differentiation of 3T3-L1 preadipocytes to adipocytes, the cells were treated with the Pluchea indica (L.) Less. tea (PIT) at various concentrations (250, 500, 750, and 1000 μg mL−1), and the intracellular lipid level was quantified using an Oil Red O staining method
The intracellular lipid accumulation showed that PIT at 750 and 1000 μg mL−1 significantly decreased the intracellular lipid accumulation to 76.87 ± 3.99 and 71.93 ± 2.05, respectively, compared to untreated 3T3-L1 adipocytes (DIF) (p < 0.05) (Figure 3)

Summary

Introduction

Obesity is caused by an imbalance of energy intake and expenditure [2]. It is necessary to treat obese individuals by encouraging a reduced calorific intake, inhibiting pancreatic lipase and adipocyte differentiation, and stimulating energy expenditure by increasing physical activity, and by regulating lipid metabolism and the surgical option such as laparoscopic adjustable gastric banding (LABG) in morbid obesity [2, 5, 6]. Reducing fat digestion and absorption can be effective in treating obesity [2]. Glycerol and fatty acids are regarded as the end products of lipid digestion in the gut; the inhibition of pancreatic lipase may be considered as a fat reducing absorption therapy [7, 8]; that is one mechanism of obesity treatment [9]

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Conclusion