The effect of non-thermal plasma Jet on bacterial biofilms and plasmid DNA

Abstract

The effect of non-thermal discharge plasma (DBD) on the inhibition of both gram-positive and gram-negative biofilms bacteria was examined at different plasma exposure times and gas flow. This effect induces damage to aqueous plasmid DNA. It reveals inactivation in bacterial biofilms for both types of bacteria with an increase in time of direct exposure to plasma and an increase in gas flow. The presence of positive bacteria outweighs the negative bacteria in susceptibility to inhibition. The resulting fractions of the DNA indicate whole DNA double-strand breaks and were determined using agarose gel electrophoresis. The damage level induced in the plasmid DNA is also enhanced with increased plasma irradiation time.