Abstract
Abstract This study was focusing on the effectiveness of microalgae harvesting method such as coagulation, centrifugation and immobilization in order to obtain high content of biomass and lipid production. The effectiveness of the three harvesting methods was determined by comparing the total amount of biomass and lipid. The procedures of this experiment comprises of (i) media preparation, (ii) microalgae cultivation, (iii) biomass harvesting and (iv) extraction of oil to obtain the lipid. The further process of converting microalgae oils into biodiesel fuel is followed by transesterification process. The lipid content and oil yield shows different amount for each different harvesting method. High total lipid content of 0.0265 + 0.0040 g and oil yield of 50.42% were obtained for centrifugation method compared to coagulation and immobilization. The fatty acid compositions of microalgae Chlorella vulgaris were analyzed using gas chromatography mass spectrometry (GC-MS) analysis for all harvesting methods. The main components were consist of palmitic acid (C16:0), stearic acid (C18:0), linolenic acid (C18:3), oleic acid (C18:1) and linoleic acid (C18:2). Among the three methods, centrifugation yields the highest amount of lipid and fatty acid methyl esters (FAMEs). Therefore, centrifugation was selected to be the most efficient method for microalgae harvesting.
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