The Effect of Marigold Leaf Ethanol Extract on Inhibin B Mice Exposed to Cigarette Smoke

Abstract

Cigarette smoke can cause oxidative damage because the amount of free radicals (oxidants) generated is far greater than the endogenous antioxidant capacity of cells. Inhibin B is used as an indicator of reproductive function in men. The content of antioxidants present in marigold leaves (Tagetes erecta), can maintain reproductive function. This study aims to analyze differences in levels of inhibin B resulting from the administration of marigold leaf ethanol extract (EDM) to mice (Mus musculus) exposed to cigarette smoke. This investigation is a laboratory experimental study with a randomized posttest-only control group design. There were 40 mice divided into 5 groups (8 mice/group). K- was only given a placebo, K+ was given a placebo and exposed to cigarette smoke 1 cigarette per day, P1 was exposed to cigarette smoke 1 cigarette per day + EDM 0.25g/kg-BW, P2 was exposed to cigarette smoke 1 cigarette per day + EDM 0.5 g/kg-BW, P3 was exposed to cigarette smoke 1 cigarette per day + EDM 1.0 g/kg-BW. The results showed that there was a significant difference between groups in the inhibin B variable with a p-value of 0.000. The results of this study showed that there was a significant difference in inhibin B levels between the K+ group with P1, P2, and P3, and the K- group with P1, P2, and P3. The average levels of inhibin B K- 0.672 ng/L; K+ 0.615 ng/L; P1 0.340ng/L; P2 0.299 ng/L; P3 0.284ng/L. The findings of this research indicate that administering an ethanol extract derived from marigold leaves to mice subjected to cigarette smoke leads to a decrease in inhibin B levels in spermatogenesis.