The Effect of Kebar Grass (Biophytum petersianum) Extract to Histopathological Images of Lactating Mice’ (Mus musculus) Kidney Exposed to Carbofuran

Abstract

This study aims to determine the effect of Kebar grass extract administration to reduce kidney damage in lactating mice exposed to carbofuran. Forty-two lactating mice were used in this study divided into seven groups, namely: K as a control group (treated with Aquadest), P1 was injected with carbofuran ¼ LD50 (0.0125mg/day), P2 was injected with carbofuran 1/8 LD50 (0.00625mg/day), P3 was injected with carbofuran 1/4 LD50 (0.0125mg/day) and Kebar grass extract (0.135mg/day), P4 was injected with carbofuran 1/8 LD50 (0.00625mg/day) and Kebar grass extract (0.135mg/day), P5 was injected with carbofuran 1/4 LD50 (0.0125mg/day) and vitamin C (0.2ml/day) and P6 was injected with carbofuran 1/8 LD50 (0.00625mg/day) and vitamin C (0.2ml/day) for 14 days. Then on the 15th day, the mice were sacrificed and the kidneys were collected, then histological preparations were made with HE (Hematoxylin-Eosin) staining. Data on tubular degeneration, necrosis, and inflammation were analyzed using the Kruskall Wallis test and after that the Mann Whitney test using the Statistical Product and Service Solutions (SPSS) application version 23 was conducted. The results showed that carbofuran caused a significant increase in tubular degeneration, necrosis, and inflammation (p<0.05). Administration of Kebar grass and vitamin C significantly reduced tubular degeneration, necrosis, and inflammation (p<0.05). It can be concluded that administration of Kebar grass extract was more effective to reduce kidney damage in lactating mice exposed to carbofuran compared to vitamin C (p<0.05).