The effect of K+ on the recovery of the twitch and tetanic force following fatigue in the sartorius muscle of the frog, Rana pipiens.

Abstract

The goal of this study was to investigate how an increase in the extracellular K+ (K0+) concentration immediately after fatigue affects the recovery of the resting potential, the twitch and tetanic contraction of frog sartorius muscle to further understand the role of K+ in the mechanism of fatigue. Resting potentials were measured with conventional microelectrodes. Twitch and tetanic contractions were elicited by field stimulation. All muscles were fatigued with tetanic contractions at a rate of one contraction per second for 3 min while being exposed to 3 mmole l-1 K0+. During fatigue development the resting potential decreased by 16 mV (control group and pH0 7.2, extracellular pH), while the decrease in the twitch force was 32.8%, compared to 79.3% for the tetanic force, and 84.6% for the maximum rate of force development of the tetanus. Fatigued muscles were also unable to maintain a plateau phase during a tetanus: force declined by 14.8% during this phase. During the recovery period under control conditions (3 mmole l-1 K0+), all four parameters returned to their pre-fatigue values, the recovery of the plateau phase was the fastest (10 min), while that of the twitch force was the slowest (80 min). When K0+ was increased to 7.5 or 9.5 mmole l-1 immediately after fatigue, the recovery rate of the tetanic force and plateau phase was reduced. The maximum rate of force development of the tetanus, however, recovered at a faster rate than control muscles. The recovery of the twitch force was also increased above that of control when K0+ was increased to 9.0 mmole l-1 (a concentration which maximally potentiates the twitch force of unfatigued muscle). Frog sartorius muscles were also tested at pH0 6.4, a pH0 which inhibits force recovery. At that pH0 the effects of K0+ were similar to those observed at pH0 7.2. It is concluded that the role of K+ in muscle fatigue is more complex and may not involve just a contribution to the decrease in force during fatigue development, but may also contribute to an increase in force development under some conditions.