The Effect of Egg Yolk Level in Skim Milk Diluent to The Motility and Fertility of Pelung Rooster Spermatozoa

Abstract

The purpose of this research was to determine the effect of the addition of egg yolk level in skim milk diluent on the motility and fertility of Pelung rooster spermatozoa. The study used a completely randomized design with 4 treatments. The treatments were P0: (skim milk + 0 % egg yolk)., P1: ( skim milk + 5% egg yolk)., P2 : ( skim milk + 10% egg yolk)., P3 : ( skim milk + 15% egg yolk). Analysis of variance results showed that the addition of egg yolk in skim milk had a highly significant effect (P<0.01) on the motility of Pelung rooster spermatozoa. The percentage of motility produced by P0, P1, P2, and P3 treatments is above 70% so it is still suitable for IB. The best treatment was P3 (skim milk + 15% egg yolk) on the motility of spermatozoa forming linear lines with the equation y = 0.61x + 76.74 and the coefficient of determination (R2) of 0.70. While Analysis of variance results showed that the level of egg yolk in skim milk diluent had a highly significant effect (P<0.01) on the spermatozoa fertility forming a quadratic line with the equation y=0.35x2 -0.64x + 6.10 and the coefficient of determination (R2=0.62). Fertility will be better without adding egg yolk. From the results of this research, it can be concluded that the addition of egg yolk level in skim milk diluent increases the motility, but not the fertility of Pelung rooster spermatozoa.
 Keywords: Pelung Rooster, Skim Milk, Egg Yolk, Motility, Fertility