Abstract
Effect of five concentrations of butylated hydroxytoluene on the percent motility and percent intact acrosomes was evaluated for bull sperm frozen and thawed in whole and skim milk diluents. Twelve ejaculates were frozen in .5-ml straws in moving nitrogen vapor after each ejaculate was split among the five butylated hydroxytoluene treatments within the two diluents. Prefreeze and postthaw percent motility and postthaw percent intact acrosomes were evaluated at 0 and 4h. Before freezing, sperm motilities were similar in whole or skim milk diluents with and without butylated hydroxytoluene. Upon thawing, sperm motility was 10 percentage points higher in whole milk diluent containing .5 and .75mM butylated hydroxytoluene than in samples without it. After 4h, percentage motile sperm was similar among all whole milk treatments. Sperm motility was similar among all skim milk treatments at both 0 and 4h. Percent intact acrosomes were similar among all treatments for each diluent. Oxidation in whole milk diluent stored at 4°C for 1 wk was reduced in the presence of 1.0, 2.0, and 4.0mM butylated hydroxytoluene.
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