The Effect of Disinfection Methods on C. Albicans in Three Types of Denture Base Materials

Abstract

This study aimed to evaluate the efficacy of disinfectant solutions on Candida albicans (C. albicans) for different types of denture materials. A total of 144 specimens (10x10x2 mm) were obtained from three different materials: autopolymerized acrylic resin, heat-cured acrylic resin, and hard relining material (n = 6). Three disinfectant solutions were used: 100% white vinegar, 2% chlorhexidine digluconate (Saver) and denture cleaning tablets (Corega). The specimens were placed on Eliza plates and 1.5 mL of Yeast Extract Peptone (YPD) was added to each well. Then, 30�L of candida culture was added to the wells. Next, the specimens were incubated at 37�C at 80 rpm for 48 h. Disinfectants were added to the Eliza plates. For all specimens, the disinfectants were replaced with 2 mL of sterile water and kept at 100 rpm for 30 min. Then, 0.1 mL of the liquid was taken and inoculated into the pads containing YPD medium. After incubation, the candida colony growth on the pads was measured. Scanning electron microscope (SEM) images were taken from randomly selected specimens from each group. Statistically significant differences (𝑃 [ 0.05) were found between the disinfectant method groups and the control group for the three types of denture materials. The 2% chlorhexidine gluconate (Saver) disinfectant was the most effective for C. Albicans ATCC 60193 and oral isolate of C. Albicans for all three of the tested denture materials. The effect of cleaning of tabs (Corega) and white vinegar was found to be similar.