Abstract

Objective. The aim of this study was to evaluate alternative methods for the disinfection of denture-based materials. Material and Methods. Two different denture-based materials were included in the study. Before microbial test, the surface roughness of the acrylic resins was evaluated. Then, the specimens were divided into 8 experimental groups (n = 10), according to microorganism considered and disinfection methods used. The specimens were contaminated in vitro by standardized suspensions of Candida albicans ATCC#90028 and Candida albicans oral isolate. The following test agents were tested: sodium hypochlorite (NaOCl 1%), microwave (MW) energy, ultraviolet (UV) light, mouthwash containing propolis (MCP), Corega Tabs, 50% and 100% white vinegar. After the disinfection procedure, the number of remaining microbial cells was evaluated in CFU/mL. Kruskal-Wallis, ANOVA, and Dunn's test were used for multiple comparisons. Mann Whitney U test was used to compare the surface roughness. Results. Statistically significant difference (P < 0.05) was found between autopolymerised and heat-cured acrylic resins. The autopolymerised acrylic resin surfaces were rougher than surfaces of heat-cured acrylic resin. The most effective disinfection method was 100% white vinegar for tested microorganisms and both acrylic resins. Conclusion. This study showed that white vinegar 100% was the most effective method for tested microorganisms. This agent is cost-effective and easy to access and thus may be appropriate for household use.

Highlights

  • The number of elderly people worldwide has been increased by increasing lifetime [1]

  • Significant differences (P < 0.05) were found between groups of the methods (Groups 1, 2, 3, 4, 5, 6, and 7) and control group (Group 8) for heat-cured acrylic resin according to both Candida albicans strains (Table 2)

  • There were statistically significant differences (P < 0.05) between groups of the methods (Groups 1a, 2a, 3a, 4a, 5a, 6a, and 7a) and control group (Group 8a) for autopolymerized acrylic resin according to both Candida albicans strains (Table 2)

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Summary

Introduction

The number of elderly people worldwide has been increased by increasing lifetime [1]. This condition resulted in a high prevalence of edentulism and complete denture wearers [2, 3]. Oral cavity is colonized by various pathogens and this microbial reservoir can cause several infections including denture stomatitis, aspiration pneumonia, and lung and gastrointestinal infections [5]. Denture stomatitis is possible source of infections in especially immunosuppressed patients [6]. Mechanic and chemical methods are frequently advised for denture hygiene. Some individuals fail to keep oral and denture hygiene because of limited motor capacity, so biofilm accumulation occurred [7]. Arendorf and Walker [8]

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