The effect of Centella asiatica ethanolic extract on expression of glucose transporter 1 and osteocalcin on stunting larvae of Zebrafish (Danio rerio)

Abstract

Stunting is known as length for age below minus two standard deviations (<-2 SD) of the WHO Child Growth Standard nutritional status. Several causes are identified, including exposure to pesticides (rotenone), which works by inhibiting mitochondrial complex I as an ATP-producing site and generate oxidative stress that affects glucose delivery and bone elongation involving GLUT 1 and osteocalcin. Centella asiatica is a high antioxidant herbal plant with anti-inflammatory properties. This study aimed to investigate the effect of Centella asiatica ethanolic extract on the rotenone-induced stunting zebrafish (Danio rerio) larvae model through GLUT 1 and osteocalcin expressions. This study found that 12.5 ppb of rotenone can induce about 6 dpf (day post-fertilization) zebrafish larvae into stunting. The effect of Centella asiatica ethanolic extract on increasing body length in stunting zebrafish larvae works by increasing the expression of GLUT 1 and osteocalcin expressions. The correlation test showed a positive and strong relationship between this ethanolic extract level with GLUT 1 and Osteocalcin expression. Thus, the Centella asiatica ethanolic extract effectively promotes the body length by elevating the expression of GLUT 1 and osteocalcin in stunting zebrafish larvae.