Abstract
Objective To investigate the changes of cytokines in rats with Clonorchiasis and the protective effects of Milkvetch Root on liver tissue.Methods Adult 32 male rats were randomly divided into four groups:control group,model group,Albendazole (ALB) group and Albendazole combined with Milkvetch Root (ALB + MR) group (8 rats / group).The experimental rats were infected with 50 metacercariae of C.sinensis or equal physiological saline through a gavage needle.The ALB group and ALB + MR group received ALB (50mg/kg/d) orally for 5days,or with MR intraperitoneally (800 mg/kg/d) for 30 days.All rats were sacrificed after 14 weeks of infection.Histopathological examination of rats' livers was performed.The levels of IL-2 and IL-4 in the serum of the rats were detected by ELISA assays.Results The vacuolization of hepatocytes and infiltration of white cells were observed in the model group,but did not observed in control group under light microscope.The injury of liver tissue of rats in ALB + MR group was milder than ALB group.The level of IL-4 in serum of rats in model group increased significantly(F =10.44,P =0.003 4),but the level of IL-2 decreased significantly compared with control group (F =18.3,P =0.000 2).The level of IL-4 in serum of rats in ALB group and in A LB + MR group decreased significantly (F =9.69,P =0.0046 ; F =7.61,P =0.010 7),while the level of IL-2 of rats in ALB group and in ALB + MR group increased significantly (F =8.69,P =0.006 8 ; F =5.5,P =0.027 3).Conclusion Our results suggested that IL-2 and IL-4 were involved in the pathogenesis of clonorchis sinensis.Milkvetch Root could provide protection to the liver tissue against Clonorchis sinensis,which might associate with the regulation of disturbed cytokines. Key words: Clonorchis sinensis ; Cytokine ; Milkvetch Root; Albendazole
Published Version
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