Abstract
1. 5-Methyltryptophan inhibits the synthesis of protein by Escherichia coli. In “stringent” but not in “relaxed” strains there is a similar reduction in the rate of synthesis of RNA. This difference is discussed in relation to current ideas concerning the regulation of RNA synthesis. 2. Ribosomes and neosomes (the immediate precursors of ribosomes) are synthesized by both stringent and relaxed strains in the presence of the amino acid analogue. 3. In addition, relaxed organisms accumulate particles sedimenting at about 15 S, a phenomenon that is characteristic of situations in which considerable differential synthesis of RNA occurs. 4. The overproduction of RNA in a relaxed strain is accompanied by preferential synthesis of ribosomal protein. 5. RNA derived from the 15-S particles accumulated by the relaxed strain in the presence of 5-methyltryptophan differs slightly from RNA isolated from ribosomes.
Published Version
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