Abstract
Haploid wild-type cells of both exponential and stationary phase cultures of Saccharomyces cerevisiae have been shown to remove from the nuclear DNA only a small percentage of the pyrimidine dimers induced by an incident dose of 5000 ergs/mm 2 of ultraviolet light on their post-irradiation incubation in nutrient medium. This removal is enhanced for late exponential and stationary phase cells by a post-irradiation incubation in saline prior to incubation in nutrient medium. Changes in the detectable amounts of such ultraviolet-induced pyrimidine dimers brought about by the application of photoreactivating light following all the forementioned post-irradiation treatments were also monitored. In all instances the cell survival was estimated, an attempt being made to correlate the biochemical and physiological data obtained.
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