Abstract
A rapid and sensitive HPLC method has been developed for the quantitative analysis of guanethidine and N-oxide in biological fluids. A reverse-phase HPLC method was employed, using an ODS stationary phase, with 55% 0·15 M HCl and 45% methanol as mobile phase. The guanidino compounds were derivatized by reaction with 9,10-phenanthraquinone and detected by UV spectrophotometry at 320 nm. Sensitivity to 3 μg mL−1 was demonstrated. We believe this to be the first reported method to employ UV detection for the analysis of guanethidine.
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