The determination of rubella hemaogglutination inhibition antibodies protective concentration with RV-IgG standard

Abstract

Objective To determine the rubella hemaogglutination inhibition (HI) antibodies concentration in persons with rubella vaccine immune serum by RV-IgG standard-based quantitative ELISA and HI test,and verify the accuracy of the kit.Methods RV-IgG standard which was diluted to eight different concentrations,and 10 pairs of serum samples before and after immunized were used to detected the rubella virus serum antibody by quantitative ELISA test,respectively.The hemagglutination tests were performed with rubella virus haemagglutinin which were crudely extracted.The hemagglutination inhibition tests were respectively proceeded with RV-IgG standard and serum samples.After the comparison of the results,rubella antibody protective concentration was confirmed.Results There was no significant difference ( t =2.151,P ＞ 0.05 ) between the measured concentration and the known concentration of RV-IgG standard diluted by the quantitative ELISA test.Comparing 10 pairs of the serum antibody level before and after vaccination,the concentration of serum antibody was significantly increased,P ＜ 0.05.Rubella virus hemagglutinin agglutination titer was 1 ∶ 128,RV-IgG standard hemagglutination inhibition titer was 1∶32.The corresponding antibody concentration was 25 IU/ml Conclusion Our results confirm that the protective concentration of rubella virus antibody is ≥ 25 IU/ml;Ater primary immunization of rubella vaccine,the serum antibody positive conversion rate can reach 90％. Key words: Rubella virus; RV-IgG standard; Hemagglutination inhibition test; Enzyme-linked immunosorbent assay