Abstract
Objective To compare the sensitivity and specificity for detecting serum anti-rubella IgG antibody between micro-hemagglutination inhibition ( HI) test and enzyme-linked immunosorbent assay ( ELISA) , and define the feasibility of ELISA for detecting serum anti-rubella IgG antibody. Methods Serum samples from 506 healthy children aged 8-15 months in Jiangsu Province were collected before vaccination and one month after vaccination with measles-mumps-rubella combined vaccine, and total 1012 serum samples were obtained. Serum anti-rubella IgG antibodies were detected by HI test and ELISA, respectively, and the detection results between two methods were compared by chi-square test. Results The positive detection rates of HI and ELISA antibodies were 50.30% and 47.04% , respectively, and no statistically significant difference in positive detection rate was found between the two methods (X2 = 1. 780, P =0. 182). Four hundred and ninety-five serum samples were negative and 476 serum samples were positive by both assays. The coincidence rate of detection between two methods was 95.95%. Correlation and regression analysis showed there was high positive correlation between two methods(r=0. 806,P<0.001). Conclusion ELISA can replace HI test to detecting serum anti-rubella IgG antibody. Key words: Rubella virus; Antibodies; Hemagglutination inhibition tests; Enzyme-linked immunosorbent assay
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