Abstract
The kinins, a group of biologically active polypeptides, are generally considered as important chemical mediators in the inflammatory process [l-3]. However, information is lacking for the role of these mediators in periodontal diseases, due to the lack of a suitable analytical method of determination. Several methods that have been reported for the determination of kinins in saliva and gingival fluid were based on a biological assay using guinea pig ileum or rat uterus [4]. However, the biological reactions involved were not specific to kinins so that the values obtained from these methods were not accurate. The present work was attempted in order to establish a method for the analysis of bradykinin (BK) in human saliva without employing a biological reaction. We have developed an HPLC method which does not require any complicated anatomical technique nor any biological reaction but which was able to specifically quantify BK.
Published Version
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