スケーリングとブラッシングによる歯周ポケット浸出液中の Bradykininase の消長

Abstract

Kinins are generally considered as important chemical mediators in inflammatory process. However, the role of these mediators in periodontal disease still remains unknown. Recently, Omori and Mizuno measured bradykininase activity in gingival fluid of the patients with periodontal disease and compared it with various clinical findings. They showed that this enzyme had a marked correlation with PMA-Index score (P<0.01), plaque score (P<0.001), calculus score (P<0.01) and pocket, depth (P<0.001).The present study was performed to know the effect of scaling and brushing on both the variation of bradykininase activity in pocket exudate of the patients with periodontal disease and their clinical findings.Twenty seven patients with periodontal disease (10 males and 17 females, aged 16 to 46) were examined in this study. The subjects were separated in two groups, the first group having only a scaling and the second group receiving a scaling and a brushing. The pocket exudate was collected from periodontal pocket by filter paper strips just before and a week, two weeks and three weeks after the treatment. After the strips were weighted, they were dipped in the inhibitor (Trasylol) and then homogenized by Polytron homogenizer. The mixture was centrifuged at 2, 500g for 20min. at O°C and the supernate was used as assay. The bradykininase activity was determined by measuring the amount of pocket exudate (mg) which inactivated 50% of added 1μg/ml bradykinin (biological method using guinea pig ileum)The results of study showed that PMA index, plaque index, pocket depth and exudate quantity decreased more significantly in scaling and brushing group than in only scaling group.In both groups, the bradykininase activity in pocket exudate had a tendency to increase with improvement of periodontal condition, paticularly in scaling and brushing group.