Abstract
Yersiniosis is an important zoonosis, causing disease to humans and animals. The available culture methods for detecting Y. enterocolitica in the samples prelevated from different categories of biological products need more time for performing and sometimes they offer satisfying results. By using methods based on DNA detection, as molecular techniques, this pathogenic agent can be detected quickly and with a higher accuracy. The purpose of this study was to establish a quicker and more veracious PCR method for the detection of this microorganism in biological product samples. There has been used a nucleotide target sequence for the PCR test, 5’ nuclease, the chromosomal gene ail, present in pathogenic strains of Y. enterocolitica. The sample was marked at 5’ end with a report dyeing substance and at 3’ end with an extinction substance. The reading was performed by RT-PCR, using a 2 stage-work protocolem consisting of 45 cycles. This qualitative PCR method demonstrated the procurement of specific results and with a high sensitivity, the detection rate of ail positive gene of Y. enterocolitica in 140 samples of biological products being of 86 % by using PCR and 37 % for culture methods.
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Schedule a callMore From: Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca Animal Science and Biotechnologies
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