Identification and characterization of Yersinia enterocolitica strains isolated from pig tonsils at slaughterhouse in Central Italy.

Abstract

Yersinia enterocolitica causes foodborne disease in humans and infections are usually acquired from contaminated raw or undercooked pork. Pigs are considered the primary reservoir of human pathogenic bio-serotypes. A total of 376 tonsil tissue samples collected after evisceration and cutting from pig carcasses were tested for Yersinia enterocolitica. Animals came from an abattoir located in the Abruzzo region, Italy. Yersinia enterocolitica was isolated from 35 out of 376 (9.31%) samples. A total of 47 strains were isolated, the prevalent bio-serotype was 4÷O:3 (95.74%), followed by bio-serotype 4÷O:9 (2.13%), and 3÷O:9 (2.13%). When characterized by DNA microarray, all strains clustered into 2 main groups. The bigger group was characterised by the presence of plasmid genes of the secretion apparatus as well as by the genes for the agellum transport machinery, while the smaller group was characterised only by genes for the agellum transport machinery. The high frequency of the pathogenic biotype 4÷O:3 able to infect humans and considered an emerging zoonotic pathogen con rms the role of pigs as natural reservoir. Since there is no official data on Yersinia enterocolitica, it is difficult to assess the implications of this food pathogen for public health. A monitoring program should be implemented for contamination in food in order to assess the risk for the consumer linked to raw or undercooked pork products.