Abstract
Dendritic cells (DCs), loaded with tumor antigens, are widely investigated as a potential therapeutic tool for cancer treatment. CCR7 plays the key role in DCs migration to lymph nodes for the anti-tumor immune response activation. The purpose of this study was to investigate the effect of different compositions for DC maturation on the CCR7 expression. Materials and methods. DCs were derived from peripheral blood monocytes in medium supplemented with GM-CSF and IL-4. Five different compounds were used for stimulation of DCs maturation. The expression of CCR7 was evaluated by flow cytometry. Results and discussion. The highest level of CCR7 expression was seen when the ‘‘cytokine cocktail” (TNF+IL-1+IL-6+PGE2) or the poly I:C were used for the stimulation of cell maturation. At the same time, the immunosuppressive effect of PGE2 and poly I:C cannot be excluded. Therefore, additional investigations of functionality of DCs after induced maturation are required.
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