The correlation study between the distribution of two single nucleotide polymorphism（SNP）loci on chromo-some 9p21 and the macrovascular disease in Xinjiang Uygur and Han nationality people with type 2 diabetes

Abstract

Objective To assessed the distribution of two single nucleotide polymorphism(SNP)loci (rs2383206.rs10757278) on chromosome 9p21 in Xinjiang Uygur and Han nationality populations, and to investigate correlation and the incidence of all cases of macrovascular disease(coronary artery disease, carotid atherosclerosis and peripheral arterial disease) and analysis of risk factors. To further study the correlation between the incidence of two single nucleotide polymorphism(SNP)loci(rs2383206.rs10757278)on chromosome 9p21 in type 2 diabetesmelli-tus(T2DM)of Han and Uygur ethnic and the incidence of all cases vascular disease, then to analysis the risk factors. Methods 497 adults with T2DM who were treated in the Endocrinology department in hospital from May 2012 to April 2014 were involved in this study, including 298 Uygur patients and 199 Han patients. 215 non-T2DM patients who were treated in the Cardiology department in hospital were also involved in the study, including 93 Uighur patients and 122 Han patients. Then the total 712 patients were detectedby using PCR-SNP Stream technology to analyse rs2383206.rs10757278 loci SNP genotyping. The relevant results were compared with t test, two different genotype distribution and allele frequency were compared with χ2 test, multiple factors analysis were calculated by Logisitic regression. Results The distribution of genotype with two SNP loci had no significant difference between the patients in Uygur group and Han group(rs2383206χ2＝5.570,P＝0.062;rs10757278χ2＝2.721,P＝0.257), and there's no significant difference between the patients with macrovascular disease and non-macrovascular disease in all patients(rs2383206χ2＝0.120,P＝0.950; rs10757278χ2＝1.027,P＝0.598). Logisitic regression analysis showed that the incidence of macrovascular was significantly associated with increasing age(χ2＝28.820,P＝0.000)and fatty liver(χ2＝5.210,P＝0.020)in Uighur group with type 2 DM. In Han group with type 2 DM, the macrovascular was significantly associated with the increase of age(χ2＝19.980,P＝0.000), elevated fasting blood glucose (FPG)(χ2＝4.070,P＝0.044)and poor controlled with glycosylated hemoglobin(χ2＝4.280,P＝0.040). Conclusion This study found that there's no correlation between two single nucleotide polymorphisms(SNPS)loci (rs2383206.rs10757278)on chromosome 9 p21 large with macrovascular in Uygur group and Han group. Increasing age, higher FPG and poor controlled with glycosylated hemoglobin combined with fatty liver were the risk factors for macrovascular. Key words: 9p21single nucleotide polymorphism genes; Macrovascular; Diabetes mellitus, Type 2