The correlation between urinary albumin excretion rate and the level of serum miR-130b in patients with type 2 diabetes mellitus

Abstract

The aim of this study was to detect the levels of serum miR-130b expression in patients with type 2 diabetes mellitus and to analyze their correlation with diabetic renal damage.243 patients with type 2 diabetes mellitus were divided into three groups according to urinary albumin/creatinine ratio(UACR): normoalbuminuria group(UACR 300 mg/g, n=54). The levels of serum miR-130b were validated by realtime polymerase chain reaction. Serum transforming growth factor-β1(TGF-β1)and tumor necrosis factor-α(TNF-α)were determined by enzyme-linked immunosorbent assay(ELISA)in all patients and 59 healthy volunteers. Compared with control group, the level of serum miR-130b in the type 2 diabetes mellitus group were significantly decreased, gradually with the increases of UACR.The level of serum miR-130b was inversely correlated with blood urea nitrogen(r=-0.295, P<0.05), serum creatinine(r=-0.316, P<0.05), UACR(r=-0.463, P<0.05), but positively related to the estimated glomerular filtration rate(r=0.367, P<0.01). The level of serum miR-130b was also negatively correlated to homeostasis model assessment for insulin resistance, triglyceride, low density lipoprotein-cholesterol, TNF-α, and TGF-β1(r=-0.257, -0.345, -0.242, -0.562, -0.622, all P<0.01). The present study indicates that serum miR-130b might be a potential new biomarker for early diagnosis of diabetic nephropathy. Serum miR-130b might be involved in the pathogenesis of diabetic nephropathy. (Chin J Endocrinol Metab, 2016, 32: 220-223) Key words: Diabetes mellitus, type 2; Serum miR-130b; Diabetic nephropathy