Abstract
Objective To investigate application values of Liquid-based cytology(TCT), cell block and immunohistochemical on estimating sources of malignant tumor cells in hydrothorax. Methods Collect 113 cases of Pleural effusion by Liquid-based cytology that are tentatively diagnosed as malignant tumor or suspected malignancies from January 2013 to June 2016 in Yuncheng Central Hospital. Through centrifuging to collect sediment cells and make paraffin block, 99 cases are selected for immunohistochemical examinations, through the CK7,CK20 and villin immunohistochemical staining determine the direction of the source of tumor, then targeted choose the TTF-1,NapsinA,CK5/6,p63,CR,Syn,CgA,CA125,GCDFP- 15,ER,PR and CDX-2 antibody to immunohistochemical staining. Results Among 94 cases of adenocarcinomas by TCT, 92 cases are still diagnosed as adenocarcinomas by immunohistochemistry. In addition, tumours sources of 92 cases are as following: 70 cases are from lung, 2 cases are from breast, 6 cases are from ovary, 8 cases are from alimentary canal, 1 case is from peritoneal serouspapillary carcinoma, and 5 cases are unknown sources. The other 2 cases are definitely diagnosed as reactive mesothelial cells and tumors of mesothelium respectively. The same diagnostic results of squamous-cell carcinoma and small cell carcinoma by TCT, cell block and Immunohistochemistry are 2 cases and 3 case respectively. The sensitivity and idiosyncrasy of Immunohistochemistry on determining the direction of tumours sources are 94.6% and 100% respectively, which are better than histological examination. Conclusions Making hydrothorax into cell blocks for immunohistochemical examination not only save tumor cells in hydrothorax, but also gathering and collecting tumor cells by centrifuge improves positive detection rate of cellular pathology and contributes to differential diagnosis of tumour cell, analysis of tissues sources, gene mutations detection and molecular targeted therapy. Therefore, it is significant to prolong patients'lives, lessen their pain, improve living quality and clinical values. Key words: Liquid-based cytology; Cell block; Immunohistochemica; Tumor cell origin
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