Abstract
Purpose: Colorectal cancer is known as the most common gastrointestinal cancers. As the age increases, the risk for this cancer also increases, so the only way to improve and hope for life in these patients is early diagnosis of the disease. So far, numerous molecular studies have been carried out on microRNAs in colorectal cancer. In addition, since some of them can be identified as cancer biomarkers. Therefore, in this study we have investigated the expression level of Mir-30d and Mir-181a as cancer biomarkers.
 
 Method: The changes of Mir-30d and Mir-181a expression levels in 60 colorectal tumor tissues and 60 adjacent tumor tissues, after RNA extraction and cDNA synthesis were surveyed using the Real Time-PCR method.
 
 Results: The results have reported a considerable reduction in the expression level of Mir-30d in tumor tissues, as well as a significant increase in the expression level of Mir-181a tumor expression in tumor tissues (P<0.05). In addition, the correlation between Mir-30d and Mir181a showed that there was a significant difference between the level of expression of mir-30d with age and TNM stage of CRC (P<0.05), whilst these correlations were not observed for Mir-181a (P>0.05).
Highlights
Third cancer worldwide is related to colorectal cancer (CRC)
The results obtain of Mir-30d expression in 60 normal and tumor tissues demonstrated that there is a significant difference between the expression level of this microRNA in tumor tissues compared with normal tissues (P=0.0014)
We analyzed the expression level of Mir-30d in 60 colorectal cancer patients and we demonstrated that Mir-30d was downregulated in CRC tissues compared to control group
Summary
Third cancer worldwide is related to colorectal cancer (CRC). This type of cancer in patients is often diagnosed in advanced stages, so that treatment for these patients in early cases is Surgery (De Rosa et al, 2015). Colorectal cancer is a very heterogeneous disease. Familial Colorectal Cancer is a group of diseases that do not follow Mendelian inheritance. The incidence of this disease in men is 30-40% higher than in women (Siegel et al, 2017). In 2018, colorectal cancer accounted for about 6.1% of the world’s total cancer mortality, and after lung cancer and prostate cancer, was the most common cancer in men (Bray et al, 2018). That of 95,520 cases, 47,700 cases of colon cancer related to men and 47,820 cases related to women
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