The cell response to the effect of heliogeophysical factors and extremely high frequency radiation of low intensity

Abstract

The aim of the work was to investigate the effect of low-intensive electromagnetic radiation (EMR) of extremely high frequencies (EHF) on the metachromasia reaction of yeast Saccharomyces cerevisiae depending on heliogeomagnetic environment, and to determine the effect of phosphates in the culture medium on the cell response. The culture Saccharomyces cerevisiae Y-517 was grown on the solid nutrient medium made on the base of wort. The media Sabouraud (without phosphates) and Sabouraud+ (with addition of phosphates) were also used. The yeast was reseeded daily, the smears were prepared, stained with methylene blue, the type of color was determined using microscope, color changing indicated metachromasia. Yeast cells were affected by EHF-radiation (120 μW/cm2) at 54, 65, 73 GHz. Heliogeomagnetic activity was controlled using Kp and Ap indices. The cell response to the effect of heliogeophysical factors was observed only when cultivating yeast in the medium with phosphates; it used to change under the influence of EHF-radiation, the third coloration type didn’t appear. We supposed intracellular water to be the unified target for both physical factors. Probably, destabilization of the structured intracellular aqueous medium by EHF-radiation changes the strength of the impact of heliogeophysical factors on cell macromolecules.