Abstract
Dissociated nerve cells from 7 day old chick embryo cerebral hemispheres have been cultivated in Rose chambers. These cells have been maintained in a stable differentiated state for several months in minimal nutrient medium. Brain extracts from chick embryos, and from new born rats, added to the nutrient medium during the first 2 weeks, stimulated the differentiation of neurones and glial cells. These well-differentiated nerve cells have also been maintained for very long periods in culture. From these morphological observations, it has been concluded that dissociated nerve cells cultivated in Rose chambers provide good experimental models for electrophysiological investigations.
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