Abstract
Dissociated brain cells from 7-day-old chick embryos have been cultivated in minimal nutrient medium and in the presence of 8-day-old embryo brain extract. A stimulation of the morphological differentiation of neuronal and glial cells has been observed. Active factors of molecular weight between 1000 and 15,000 (dialyzate of the brain extracts) were found to stimulate the maturation of neuronal cells, while factors of molecular weight higher than 15,000 (dialyzed brain extract) enhance the differentiation of glial elements. The dialyzable active substances are heat labile and sensitive to trypsin treatment, which suggest that they are proteins. Furthermore they are stable upon prolonged storage at −70°C.
Published Version
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