The Association of prolactin receptor gene polymorphism with recurrent miscarriage in a sample of Iraqi women

Abstract

Recurrent miscarriage (RM) is a common occurrence, about 15% of all clinically recognized pregnant women resulting in pregnancy failure. RM has been inconsistently defined. When defined as 3 consecutive pregnancy losses prior to 20 weeks from the last menstrual period, it affects about (1- 2) % of women. our study study whether the polymorphisms in PRL-R gene are correlated with the risk of RM. RM casas was (n=25) and apparently healthy control subject (n=15), were enrolled genotyping of PRL-R gene SNP (rs37389 G>T) polymorphism were determined by using Taqman genotyping assay. This study was carried out in the laboratory of institute of genetic angering and biotechnology for postgraduate study- university of Baghdad from November 2018 to February 2019, and the sample were collected from several laboratories in Baghdad. the distribution of genotypes and alleles frequencies results showed that at rs37389‎ SNP of PRL-R gene, as related with TT, TG and combined TG+GG genotypes, significant differences in frequency percentage were recorded between RM patients and apparently healthy subjects, the frequency of wild TT genotype was significantly lower in patients with RM than in apparently healthy subjects. In contrast, the frequency of heterozygous TG genotype and mutant genotype were significant higher in patients with RM compared with apparently healthy subjects. This study found an association between both heterozygous and homozygous mutants genotype at rs37389 of PRL-R gene with a risk for RM susceptibility.