The application value of sperm nuclear DNA integrity and sperm morphology in the treatment of ICSI

Abstract

Objective To investigate the effects of sperm DNA fragmentation (SDF) and sperm morphology on the fertilization and embryo development in ICSI. Methods SDF and sperm morphology were detected in the meanwhile of taking eggs in 145 ICSI treatment cycle.On the basis of SDF index (DFI) divided into group A (DFI≤30%) and group B (DFI>30%). According to the normal sperm morphology divided into group C (NMSR≥4%), group D (1%≤NMSR 30% and NMSR<4%). Statistically analyzed ICSI outcome of research group: fertilization rate, embryo utilization rate, good quality embryo rate, implantation rate and clinical pregnancy rate. Results (1)The normal fertilization rate, embryos utilization rate, good quality embryo rate, implantation rate in group A were significantly higher than those in group B (χ2=6.96, 8.95, 5.49, 3.92, all P 0.05). (2) The normal fertilization rate was statistically significant in group C, group D, group E (χ2=34.5, 65.8, 11.8, all P 0.05). (3)The normal fertilization rate, embryo utilization rate, good quality embryo rate, implantation rate and clinical pregnancy rate in group F were significantly higher than those in group G, and normal fertilization rate, embryos utilization rate, good quality embryo rate had statistically significant differences (χ2=37.5, 11.0, 4.3, all P<0.05). Conclusion Sperm abnormal morphology has negative effect on fertilization, and the high DNA fragments have negative effects on fertilization and embryo development. Key words: DNA fragmentation rate; Sperm morphology; ICSI; Embryo