The Application of DNA Barcodes in the Authenticity Identification of Marine Fishes

Xueying Zhang,Qiong Jiang,Cheng Zhu,Yanfei Din

doi:10.19026/ajfst.11.2735

Xueying Zhang, Qiong Jiang + Show 2 more

Open Access

https://doi.org/10.19026/ajfst.11.2735

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Abstract

Recently, the study about Marine fish identification is becoming a big challenge for us. Marine biodiversity is always to been underestimated for we can’t know how enormous it is. And because of the boundedness, the existing traditional taxonomic methods have not enough ability to detect the mystery of ocean and we must seek for a new way to research how many kinds of fishes in ocean and what kind of the fish is. What’s more, with the internationalization of market, not only taxonomy, but the seafood market chaos and plenty of issues about food safety also reflect the importance of Marine fish identification. In this context, a new identification tool -- --DNA barcodes has appeared. DNA barcodes is a Molecular identification methods which through sequencing the mitochondrial genes of Cytochrome c Oxidase subunit I(COI) sequence and being compared with DNA barcodes database to rapid identification of the sample. In this study, we will introduce the principle, advantages and limitations of DNA barcodes and summarize the application of DNA barcodes in the aspect of Marine fish and seafood identification. All kinds of application research show that DNA barcodes has high resolution and can easily identify fishes to species level. So we have reason to believe that the DNA barcodes can been made full use of in the field of Marine fish authenticity identification though the research of predecessors. What’s more, it benefits to enhance the effectiveness of food anti-counterfeit and can help to manage the seafood market more effectively.

Highlights

The sea is the most extensive in the world, and the most mysterious to human beings
In order to verify the reliability of DNA barcode technology, Rasmussen et al (2009) widely captured more than 1000 salmon in North America to conduct DNA barcode identification, the results showed low intraspecies divergence and the mean congeneric divergence was 8.22%, 32-fold greater than intraspecies divergence and the minimum interspecies divergence was greater than the maximum intraspecies divergence, proved the feasibility of DNA barcode on the species identification
We can found that DNA barcode technology has obvious advantages in identifying those fish which have difficult in recognizing their fuzzy morphological characteristics compared with other classification methods

Summary

INTRODUCTION

The sea is the most extensive in the world, and the most mysterious to human beings. In order to pursuit of similar taste, mouthfeel traditional classification systems underestimated the with the genuine, these food are always added all kinds polymorphism of Marine fishes. They thought that there of colloid and various additives, bringing serious harm are at least a third of the 1000 kinds of fish in South to people’s health. What’s more, there are some difficulties process of production since the diversity of the way of in morphological identification, which is widely process, we can’t use traditional taxonomy method distribution of monomorphic species in Marine systems to identify these material which have lost original form and the existence of some sexual binary and phenotypic this mean traditional taxonomy method is not plasticity species (Radulovici et al, 2010). Spectral method are commonly used (Zhou and Yuhua, 2009)

This method is identify species by analysing

Findings

CONCLUSION