Abstract
The aim of this study was to increase the Coenzyme $Q_{10}$ $(CoQ_{10})$ yield from <i>Rhodobacter sphaeroides</i> via genetic engineering pathway. $CoQ_{10}$ plays important roles in many biological processes and has been proven to be effective in the treatment of many diseases. In the present study, the <i>ubiG</i> gene located in $CoQ_{10}$ biosynthesis pathway was effectively overexpressed in <i>Rb. Sphaeroides</i> to increase $CoQ_{10}$ production. The growth of host cells was slightly influenced by overexpressing <i>ubiG</i>. The crude $CoQ_{10}$ production was enhanced by 58.31% compared to that from the control. The <i>ubiG</i> mRNA level was significantly increased compared to the wild type harboring empty vector as measured by qRT-PCR. Moreover, the crude $CoQ_{10}$ exhibited strong anti-oxidant activity as measured <i>in vivo</i> by zone of inhibition assay.
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