Abstract
Objective To analyze the protein components and the immunoreactivity of Trichinella spiralis new born larva (NBL) soluble antigens at different stages in Dali,Yunnan.Methods The sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was used to analyze protein components of the worm soluble antigens of NBL at different stages, and the Western-blot was used to analyze the immunoreactivity of the worm soluble antigens of NBL at different stages against T.spiralis infected rats.Results The results from SDS-PAGE analysis displayed that the worm soluble antigens of T.spiralis NBL on 24 h showed 36 protein bands with the range between Mr160000 and 10000,among which the 8 main bands were Mr73000,64000,58000,54000,46000,40000,34000 and 25000,respectively.The worm soluble antigens of T.spiralis NBL on 48 h displayed that 40 proteins bands with the range between 172000 and 10000,among which the 17 main bands were 120000,102000,89000,87000,79000,74000,72000,64000,58000,54000,46000,40000,34000,32000,25000,18000 and 10000,respectively.The results of Western-blot showed that 7 protein bands of the worm soluble antigens from T.spiralis NBL on 24 h reacted with the serum of rat anti-T.spiralis NBL.Whereas,14 protein bands of the worm soluble antigens from T.spiralis NBL on 48 h reacted with the serum of rat anti-T.spiralis NBL.Moreover,the negative serum had no cross-reactivity to corresponding proteins.Conclusion The worm soluble antigens of T.spiralis NBL on 24 h had 7 same protein components in comparison with NBL on 48 h.The protein components from the worm antigens of T.spiralis NBL increased during the development of the worm.Western-blot displayed that the serum of rat anti-T.spiralis NBL had stronger specific recognition to the 6 protein components from the worm soluble antigen of T.spiralis NBL both on 24 h and 48 h.Thus,these protein components had stronger immunoreactivity. Key words: Ttichinella spiralis; New born larvae; Worm soluble antigens; Protein components; Immunoreactivity
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