Abstract
Objective To establish the ELISA method for detection of anti-Trichinella antibody IgG in sera and saliva. Methods The phalanx titration was used for the selection of the ELISA experimental conditions such as the optimal coating concentrations of T. spiralis antigens including muscle larval soluble antigen (MLSA),muscle larval excretory-secretory antigen (MLESA),adult wornl soluble antigen (AWSA),adult worm excretory-secretory antigen (AWESA), the dilutions of sera and saliva, the dilutions of the goat anti-rabbit and the goat anti-human IgG conjugated with horseradish peroxidase (HRP).Sera and saliva from 20 rabbits,10 patients infected with T. spiralis were used for the sensitivity assay of the 4 antigens.while sera and saliva from 20 healthy parasite-free rabbits and persons,sera and saliva from 38 rabbits and patients infected with other parasites were detected for the specificity of the 4 antigens.Results The optimal coating concentrations of the 4 antigens were 8.0 μg/ml,6.0 μg/ml,10.0 μg/ml,9.0 μg/ml,respectively.The optimal dilutions of sera were 1:100,1:200,1:50,1:200 respectively.while saliva was used without dilution.The dilutions of the goat anti.rabbit and the goat anti-human IgG were 1:2 500 and 1:2 000.respectively.The sensitivities of the 4 antigens for detecting the sera and saliva from rabbits infected with T. spiralis were 100% and 80%-100%.respectively.The sensitivities of the 4 antigens for detecting the sera and saliva from patients with trichinellosis were 100% and 60%-80%.respectively.The specificities of the 4 antigens for detecting the sera and saliva were 81.03%,89.65%,77.59%.82.76%and 93.10%,96.55%,89.65%.91.35%respectively. Conclusion The ELISA assays for detection of anti-T.spiralis IgG antibody in serum and saliva samples were established.Saliva samples were promising in substituting serum for detection of triehinellosis when there were diffieulties to collect serum samples. Key words: Trichinella spiralis; ELIsA; Serum; Saliva
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