THE ACTIVITY OF LIPID PEROXIDATION AND ANTIOXIDANT PROTECTION OF THE MYOCARDIUM OF DIFFERENT SEX ANIMALS AT ADRENALINE DAMAGE ON THE BACKGROUND OF LIGHT DESYNCHRONOSIS

Abstract

Objtctive - to study the activity of free radical processes in the myocardium underconditions of adrenaline damage against the background of light desynchronosisdepending on sexMaterials and metods. In an experiment on mature white laboratory rats, the sexdifferences in the development of the necrotic process in the myocardium were studied onthe background of light desynchronosis. Myocardial damage caused by the intjection ofadrenaline (0.5 mg / kg, intramusculare). Concentration of lipid peroxidation metabolites(diene conjugates and malondialdehyde), the activity of superoxide dismutase, catalase,glutathione reductase, glutathione peroxidase and the content of sulfhydryl groups weredeterminedIn in the ventricular myocardium tissue. Such studies were performed 1 and 24hours after intjection of adrenaline, which corresponded to the periods of onset and peakcardiomyocytes necrosis. All animals were in condition of permanent lighting (500 LX) for10 days before modeling of myocardial necrosis. The controle group consisted of animalsthat were in the conditions of the natural cycle day/night.Results. It has been showed that more significant activation of lipid peroxidation in themyocardium caused adrenaline was in males of both group - control and at constantlighting. It was confirmed by a more intensive accumulation of LPO metabolits. Thebackground for such changes was a deficiency of antioxidant protection, especiallyglutathionereductase and glutathioneperoxidase.Conclusion. The development of the necrotic process in the myocardium at permanentlighting (10 days) occurred under conditions of a significant violation of the balance ofprooxidants / antioxidants with a predominance of lipid peroxidation processes. Theintensity of this imbalance was more significant in the myocardium of male rats, whichmay be due to a more significant melatonin deficiency.