�The accumulation of highly differentiated Mtb-specific CD4 T cells is associated with lung tissue destruction during pulmonary tuberculosis.�

Abstract

Event Abstract Back to Event ‘The accumulation of highly differentiated Mtb-specific CD4 T cells is associated with lung tissue destruction during pulmonary tuberculosis.’ Irina Nikitina1*, Rasul Amansahedov1, Alexander Panteleev1, Vitaly Ganusov2, Irina Vasilyeva1 and Irina Lyadova1 1 Central Tuberculosis Research Institute, Russia 2 University of Tennessee, United States Immunological methods are widely used for the diagnostics of M. tuberculosis (Mtb) infection. In this study we show that these methods can also be used as a means to evaluate and monitor the degree of lung tissue destruction caused by Mtb. Lung destruction is a characteristic trait of pulmonary tuberculosis (TB). It underlies disease severity, causes dissemination of the pathogen and needs to be carefully evaluated. Our earlier studies in mice have demonstrated that Mtb infection induces the differentiation of effector CD4 T cells into highly differentiated CD4+CD27- lymphocytes and that this differentiation takes place in the lungs. We hypothesized therefore that the accumulation of CD4+CD27- cells should mirror tuberculosis activity. In this study, we evaluated the percent of CD27- cells within the population of Mtb-reactive CD4 T cells (“Mtb+CD27-“ cells) in the blood of TB patients. Compared to healthy donors, TB patients had significantly higher percent of Mtb+CD27- cells (p<0.0001). Within the group of TB patients, the percent of Mtb+CD27- cells varied. Among different characteristics of TB disease (i.e., pulmonary destruction, disease duration, et al.), high degree of pulmonary destruction was the main factor associated with high percent of Mtb+CD27- cells (p<0.0001). ROC-curve analysis revealed that evaluation of Mtb+CD27- cells allows assessing pulmonary destruction (sensitivity, 89%; specificity, 74%). During the treatment, a decrease in the percent of Mtb+CD27- cells was indicative on the repair of lung destruction (p<0.01). The determination of the percent of Mtb+CD27- cells allows assessing pulmonary destruction and its repair following TB therapy. Support: NIH (#A1078899) Keywords: T-cell immunity, CD4 T cells, differentiation, Tuberculosis, Diagnosis, Differential, Flow Cytometry Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Host-pathogen interactions Citation: Nikitina I, Amansahedov R, Panteleev A, Ganusov V, Vasilyeva I and Lyadova I (2013). ‘The accumulation of highly differentiated Mtb-specific CD4 T cells is associated with lung tissue destruction during pulmonary tuberculosis.’. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00222 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 14 Mar 2013; Published Online: 22 Aug 2013. * Correspondence: Mrs. Irina Nikitina, Central Tuberculosis Research Institute, Moscow, Russia, redwings2009@yandex.ru Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Irina Nikitina Rasul Amansahedov Alexander Panteleev Vitaly Ganusov Irina Vasilyeva Irina Lyadova Google Irina Nikitina Rasul Amansahedov Alexander Panteleev Vitaly Ganusov Irina Vasilyeva Irina Lyadova Google Scholar Irina Nikitina Rasul Amansahedov Alexander Panteleev Vitaly Ganusov Irina Vasilyeva Irina Lyadova PubMed Irina Nikitina Rasul Amansahedov Alexander Panteleev Vitaly Ganusov Irina Vasilyeva Irina Lyadova Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.