Increase of Reactive Oxygen Species in Natural Killer cells from Pulmonary Tuberculosis patients

Abstract

Event Abstract Back to Event Increase of Reactive Oxygen Species in Natural Killer cells from Pulmonary Tuberculosis patients Diogo Silva1, Mónica Abreu1, Patricia Couceiro2, Margarida F. Teixeira1, Inês Ladeira3, Filipa Viveiros3, Raquel Duarte3, Helena Sá1 and Paulo Rodrigues-Santos1* 1 Faculdade de Medicina da Universidade de Coimbra, Instituto de Imunologia, Portugal 2 Center for Neurosciences and Cell Biology, Laboratory of Immunology and Oncology, Portugal 3 Centro Diagnóstico Pneumológico of Vila Nova de Gaia, Portugal Tuberculosis (TB) remains today a threat for public health and one third of the world population is infected. Although the role of Natural Killer (NK) cells in vivo was never fully understood, their capacity to lyse not only infected macrophages, but also directly M. tuberculosis demonstrates their possible role in the immune response in addition to their regulatory function over other immune cells and their presence inside granulomas. However their number is decreased and with impaired function in TB patients Reactive oxygen spices (ROS) produced by tissue-specific cells especially alveolar macrophages can be responsible for their suppressive state as happens in other diseases. In this work we intended to demonstrate the increase of ROS in NK cells of patients with pulmonary tuberculosis. We analyzed by flow cytometry the levels of oxidative stress in cells from peripheral blood of 14 TB patients with main focus in NK cells. In our work we demonstrated that not only phagocytic cells with active NADPH oxidase have an increase of ROS, but also NK cells and NKT cells. We did not find any significant statistical difference in CD8+ T cells comparing with healthy controls. Beyond expected decrease of total NK cell number and especially CD56dim CD16+ subset, we found that almost CD56dim are CD16- corresponding these cells to an increase of oxidative stress. We also demonstrated that contrary to granulocytes and monocytes, in multi-drug resistant (MDR) TB patients the levels of oxidative stress in NK cells are equally higher to persistent TB without (MDR-TB). Keywords: Tuberculosis, Pulmonary, Natural Killer cells, Reactive Oxygen Species, NADPH Oxidase, innate immunity Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Innate immunity Citation: Silva D, Abreu M, Couceiro P, Teixeira MF, Ladeira I, Viveiros F, Duarte R, Sá H and Rodrigues-Santos P (2013). Increase of Reactive Oxygen Species in Natural Killer cells from Pulmonary Tuberculosis patients. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00533 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 11 Jun 2013; Published Online: 22 Aug 2013. * Correspondence: Dr. Paulo Rodrigues-Santos, Faculdade de Medicina da Universidade de Coimbra, Instituto de Imunologia, Coimbra, 3004-504, Portugal, paulo.santos@fmed.uc.pt Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Diogo Silva Mónica Abreu Patricia Couceiro Margarida F Teixeira Inês Ladeira Filipa Viveiros Raquel Duarte Helena Sá Paulo Rodrigues-Santos Google Diogo Silva Mónica Abreu Patricia Couceiro Margarida F Teixeira Inês Ladeira Filipa Viveiros Raquel Duarte Helena Sá Paulo Rodrigues-Santos Google Scholar Diogo Silva Mónica Abreu Patricia Couceiro Margarida F Teixeira Inês Ladeira Filipa Viveiros Raquel Duarte Helena Sá Paulo Rodrigues-Santos PubMed Diogo Silva Mónica Abreu Patricia Couceiro Margarida F Teixeira Inês Ladeira Filipa Viveiros Raquel Duarte Helena Sá Paulo Rodrigues-Santos Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.