Abstract
Phytotoxicity effect of sodium selenite Se(IV) and selenate Se(VI) was followed on selected characteristics of garden pea ( Pisum sativum) , such as germination, growth, chlorophyll production, content of dry material and water in the seedlings. Laboratory experiments were established in the Petri dishes, which were treated with sodium selenate (Na 2 SeO 4 ) and sodium selenite pentahydrate (Na 2 SeO 3 .5 H 2 O) on the selenium concentration levels 5.0; 20.0; 100.0; 300.0 and 500.0 mg Se/l. Phytotoxicity was tested by the tests of chronic phytotoxicity and the results obtained were evaluated as IC 50 values (half maximal inhibitory concentration) by probit analysis. Treatment of seedlings with the solutions of Se(IV) and Se(VI) on the concentration levels 5 mg and 20 mg/l resulted in enhanced growth of shoots and roots, especially after application of Se(IV), where the growth exceeded control for about 70 %. The IC 50 value was higher for the growth of shoots as well as for roots after application of Se(VI), what means that the growth inhibition in early growth stages of garden pea can be observed only in the high selenium concentrations (over 200 mg Se/l). Se(IV) showed more significant inhibition of chlorophyll production in the shoots of peas seedlings than Se(VI). Lower concentrations of Se(IV) and Se(VI) (below 100 mg Se/l) did not show significant differences between the water contents, but the higher concentrations (300 mg Se(IV)/l and 500 mg Se(VI)/l) resulted in significant differences, more than 9-times higher in roots.
Published Version
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