T-CELL-MEDIATED CYTOLYSIS : FROM THE LYSIS OF H-2 NEGATIVE TARGET CELLS TO THE INDUCTIVE EFFECT OF XENOGENEIC SERUM

Abstract

Publisher Summary This chapter describes the results of a series of experiments. In the first series, evidence was found in favor of some specificity of cytolysis against embryonic antigens at the surface of the F9 cells. In a second series of experiments, it was found that P815 and EL4 cells were lysed. From a systematic investigation of the parameters of this system, two facts emerged. F9 cells were not necessary during the in vitro boost incubation. There was some preferential lysis of targets having the same H-2 as the mice receiving the H-2-less F9. For instance, if F9 cells were injected into B1O mice, and after 2–3 weeks, the spleen cells were incubated for 5 days in conventional tissue culture conditions, cytolytic cells emerged that were more efficient against EL4 cells than against P83 5 cells. The converse was observed with B10.D2 mice. It could be argued that the effect of xenogeneic serum could be similar to that of a lectin, that is, without any antigenic specificity. It was also found that more cytotoxicity was obtained in the direct combinations than in the cross combinations. This was in favor of some specificity of antigenic recognition at the level of induction by xenogeneic serum.