Abstract
This chapter presents the analysis of requirements for cell-mediated lysis interaction between viral glycoproteins and cell plasma membranes. The specificity of cytotoxic T killer cells generated in mice after infection with certain viruses is directed to antigenic determinants on cell surfaces induced by the viral and by the host genome. The target cells to be lysed have to carry antigens of the sensitizing virus and antigenic products of the K or D region of the major histocompatibility complex compatible with the specificity repertoire of the T-cells. The protease treatment abolishes the activity of both glycoproteins. Trypsin treatment inactivates fusion and hemolysis activity. V8 protease treatment inactivates HN, eliminating haemagglutinin and neuraminidase activities, but it has no effect on F as far as analysis on polyacrylamide gels, immunological analysis, and amino acid composition is concerned. Because virus–cell and cell–cell fusion has to be preceded by virus-cell adsorption, V8 protease treatment eliminates the functional activities of F as well. All three proteases block the activity of Sendai virus to form the target antigen, indicating that either haemagglutinin-neuraminidase or F is active in the process of target antigen formation.
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