Taurine Chloramine decreases cell viability and cytokine production in blood and spleen lymphocytes from septic rats.

Dhébora M Dall’Igna,Jaqueline M Da Luz,Felipe Dal-Pizzol,Monique Michels,Francieli Vuolo

doi:10.1590/0001-3765202020191311

Dhébora M Dall’Igna, Jaqueline M Da Luz + Show 3 more

Open Access

https://doi.org/10.1590/0001-3765202020191311

Copy DOI

Abstract

Taurine (Tau) is an abundant amino acid in polymorphonuclear leukocytes that react with hypochlorous acid to form taurine chloramine (TauCl) under inflammatory conditions. We investigated potential interactions between lymphocytes and TauCl in rats submitted to cecal ligation. Animals were divided into sham or CLP groups (24 or 120 h) to isolate lymphocytes from blood and spleen. Lymphocytes were cultured at a concentration of 1×106 cells/mL and activated by concanavalin A. Tau and TauCl were added at 1, 10, and 100 μM. Cells were incubated with MTT to evaluate cell viability and cytokine concentration in the supernatant was determined. TauCl decreased lymphocyte viability and altered the secretion pattern of important inflammatory mediators in non-specific-phenotype manner. The effort to a is elucidate mechanisms of immune cell (dys)function in sepsis is important to better understand the complex regulation of immune system during sepsis development, and further studies are necessary to confirm TauCl as potential target in this context.

Highlights

The Third International Consensus Definitions Task Force defined sepsis as life-threatening organ dysfunction caused by dysregulated host response to infection (Seymour et al 2016)
Cell viability alteration was more often seen at 24 hours than 120 hours both in blood and spleen in non-dependent dose manner and did not follow any specific pattern
Tau and taurine chloramine (TauCl) treatments (1, 10 and 100 μM) reduced blood cell viability after 24 hours when compared to the corresponding sham group (Figure 1a)

Summary

Introduction

The Third International Consensus Definitions Task Force defined sepsis as life-threatening organ dysfunction caused by dysregulated host response to infection (Seymour et al 2016). Polymorphonuclears (PMNs) are mostly related to innate immunity, they co-localized in T cells at sites of persistent infections, chronic inflammations or tumors (Müller et al 2009). MPO and Tau are separated in mature PMNs, the formation of TauCl inside PMNs is possible after reactive oxygen species (ROS)-driven permeabilization of azurophilic granules to the cytosol. This may occur during bacteria-induced apoptosis in PMNs Blomgran et al 2007)

Objectives

Methods

Results

Conclusion