Comparison of cytokine production in human lymphocytes and human tissue culture THP 1 stimulated by metalic ions

Abstract

Event Abstract Back to Event Comparison of cytokine production in human lymphocytes and human tissue culture THP 1 stimulated by metalic ions Zora Venclikova1*, Jirina Bartova1 and Jaromir Belacek2 1 The Institute of Clinical and Experimental Dental Medicine, Czechia 2 The institute ob Biophysics and Informatics, Czechia Introduction: Physiological interaction of foreign materials (including metallic ions) with oral mucosal tissue can lead to oral tolerance induction. However, a minority of humans induce an intense reaction to metal dental reconstruction. Cytokines produced during the interaction of lymphocytes and macrophages play an important role in activation and regulation of immune response. Aim and methods: Our study aimed at exploring cytokine production in lymphocytes of mercury and nickel hypersensitive patients (verified with a test of lymphocyte proliferation) and in a monocyte-macrophage cell line of human origin THP 1. In all cases, the cells were cultivated for five days with an addition of mercury and nickel salts. Cytokines in culture media were determined by Ray Bio® Human Inflammation Antibody Array III and compared with results of control group cells, cultivated without addition of metallic salts. Results: Lymphocytes of hypersensitive patients predominantly produced pro-inflammatory cytokines, for example IL-1 alpha, IL-11, IL-17, IFN-gamma, TNF-beta, sTNFRI, sTNFRII and TIMP-2. These cytokines were observed in both cases of metallic salts stimulation. On the other hand, cell culture THP 1 predominantly produced anti-inflammatory cytokines after stimulation with both metallic salts. The results suggested that in the case of hypersensitivity diagnosis, to follow the reaction of both lymphocytes and monocyte-macrophage cells should be recommended. Acknowledgements This study was supported by a grant from Czech Ministry of Health, IGA NT/12206-4. Keywords: Cytokines, Lymphocytes, Macrophages, protein arrays, hypersensitivity to metals Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Innate immunity Citation: Venclikova Z, Bartova J and Belacek J (2013). Comparison of cytokine production in human lymphocytes and human tissue culture THP 1 stimulated by metalic ions. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00247 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 12 Mar 2013; Published Online: 22 Aug 2013. * Correspondence: Dr. Zora Venclikova, The Institute of Clinical and Experimental Dental Medicine, Prague, 121 11, Czechia, ZVenclikova@seznam.cz Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Zora Venclikova Jirina Bartova Jaromir Belacek Google Zora Venclikova Jirina Bartova Jaromir Belacek Google Scholar Zora Venclikova Jirina Bartova Jaromir Belacek PubMed Zora Venclikova Jirina Bartova Jaromir Belacek Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.