Synthetical establishment method of fatty liver model similar with human pathophysiology

Abstract

Objective To establish animal model of fatty liver similar with human pathophysiology used for the study of imaging quantitative diagnosis or verification of medicine curative effect. Methods Forty-five New Zealand white rabbits were used in this study. They were divided randomly into five groups : 10 rabbits in normal control group, 10 rabbits in experiment group 1,10 rabbits in experiment group 2,10 rabbits in experi-ment group 3,and remaining 5 rabbits were in a supplement group. Rabbits in normal control group were given free access to normal food and water for 9 weeks. Rabbits in the experimental groups were fed a high-fat and high-sucrose diet for 3,6,and 12 weeks,respectively. Rabbits in the supplement group were fed the same diet as the experimental groups. Liver specimen stained with HE and Sudan Ⅳ was examined histological. Results The success rate of hepatic steatotie models was 87 % (39 of 45 ) ;mortality was 13 % (6 of 45 ). There were 10 nor-mal livers, 10 mildly fatty livers, 9 moderately fatty livers, and 10 severely fatty livers in results (stained with HE), and the rate of conforming to Sudan Ⅳ was 75%. Conclusion The animal model of fatty liver with differ-ent severity can be established by being fed a high fat, high sucrose and alcohol diet. Key words: Fatty liver; Animal model; Imageology study