Synthesis of methoxy substituted pyrimidine derivative imine stabilized copper nanoparticles in organic phase and its biological evaluation

Abstract

Methoxy substituted pyrimidine derivative Schiff base ligand (DMPMM) and its stabilized copper nanoparticles (DMPMM-CuNPs) were synthesized by modified Brust-Schiffrin technique. The formations of DMPMM and DMPMM-CuNPs were proved by UV–Visible and FT-IR spectroscopic studies. The size, surface morphology and the quality of DMPMM and its MNPs were analyzed by Scanning Electron Microscopy (SEM) technique. Transmission Electron Microscopy (TEM) result shows that DMPMM-CuNPs has approximately 3 to 5 nm size. Electrochemical performance of the DMPMM-CuNPs was analyzed by cyclic voltammetric method. DNA binding studies of the DMPMM and DMPMM-CuNPs with CT-DNA were inspected by four different methods such as UV–Visible, emission spectroscopy, cyclic voltammetry and viscometric measurements. Spectroscopic, voltammetric, viscometric and denaturation (Thermal and sono-chemical) studies results recommend the synthesized compounds have good DNA binding ability. The four different antioxidant scavenging studies results shows that DMPMM and DMPMM-CuNPs have significant antioxidant activity. Antimicrobial studies of the DMPMM and DMPMM-CuNPs were studied by well diffusion method. Anticancer studies results evidenced that the synthesized DMPMM-CuNPs have good selectivity to interact with human cancer cells and normal cell correspondingly. DMPMM-CuNPs destroy the normal cell line at higher concentration (84.3 μg/mL). This observation decides that the synthesized DMPMM-CuNPs has good anticancer activity. • Solid and stable copper nanoparticles were successfully prepared. • The size of the prepared copper nanoparticles was approximately 3 to 5 nm. • Prepared copper nanoparticles were soluble in most of the organic solvents. • Copper nanoparticles have good biological properties especially in anticancer studies.