Stable copper nanoparticles as potential antibacterial agent against aquaculture pathogens and human fibroblast cell viability

Abstract

The developments of green nanotechnology are generating interest of researchers towards synthesis of copper nanoparticles due to their increasing application towards the biomedical field. The utilization of phytochemicals in plant extracts have become a valuable trend in the synthesis of nanoparticles as they possess dual nature of reducing and stabilizing agents. In this work a simple and rapid biosynthesis route for producing stable fenugreek copper nanoparticles (FCuNPs) using Trigonella foenum-graecum is demonstrated and assessed its antibacterial activity against gram negative Vibrio species. The characterization of synthesized FCuNPs was carried out using UV–vis spectrophotometer and the SPR of FCuNPs is observed at 350 nm. TEM, HRTEM –SAED analysis was done to evaluate the morphology and size of FCuNPs. FTIR spectra of both the plant extract and FCuNPs were recorded in order to study the interaction of phytochemicals with FCuNPs. The antibacterial activity of biosynthesized FCuNPs was tested against V. vulnificus, V. harveyi and V. parahaemolyticus using agar well diffusion technique. Since this method of synthesizing copper nanoparticles does not involve any harmful chemicals, the FCuNPs produced are more biocompatible and were used to evaluate human skin fibroblast cell line by Alamar Blue reduction assay. The outcomes of this report will surely provide a new path in the field of nanotechnology and nano medicine where there is a significant need of antibacterial and cell viability studies. Hence, FCuNPs can be powerful therapeutic materials in numerous biomedical applications, which are to be discovered in the near prospective.