Synthesis of lipids with sn-2 palmitic acid and sn-1,3 oleic acid from hard palm stearin and oleic acid

Abstract

Human milk fat substitute (HMFS) is a fat that contains 1,3-dioleoyl-2-palmitoyl-glycerol (OPO) as the main triacylglycerol. Hard palm stearin (HPS) is a palm oil fraction containing high palmitic acid (PA), which can be used as a raw material in OPO synthesis. The challenge in OPO synthesis using HPS is to maintain sn-2 PA and increase sn-1,3 oleic acid (OA). This research was conducted to synthesize OPO through enzymatic acidolysis of the HPS-solid fraction and OA (1:6, mole ratio) using Lipozyme TL IM 10% of the substrate weight at 60 °C for 2 – 24 h. The results showed that acidolysis caused increased partial acylglycerol, decreased TAG, and decreased sn-2 PA while increased sn-2 OA and sn-1,3 OA. The presence of acyl migration caused the incorporation of OA using Lipozyme TL IM at the sn-1,3 positions after 2 h was not significantly different. The product obtained at 2 h contained TAG with 74% sn-2 PA and 17% sn-2 OA, 78% sn-1,3 PA, and 11% sn-1,3 OA.